Resolusi dari sistem modulasi cahaya tergantung dari kloroplas kacang *1 * 1 Pembelian teks-artikel penuh

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Anthony R. Ashton Anthony R. Ashton ** and Louise E. Anderson ** dan Louise E. Anderson *** ***

Department of Biological Sciences, University of Illinois at Chicago Circle, Chicago, IL 60680, USA Departemen Ilmu Hayati, University of Illinois di Circle Chicago, Chicago, IL 60680 USA
Received 6 April 1981; Diterima 6 April 1981;
revised 7 August 1981. revisi 7 Agustus 1981.
Available online 29 January 2003. Tersedia online 29 Januari 2003.

Abstract Abstrak

The mechanism of the light-dependent inactivation of glucose-6-phosphate dehydrogenase and the light-dependent activation of NADP + -malate dehydrogenase has been studied in partially purified extracts of pea ( Pisum sativum ) chloroplasts. Mekanisme inaktivasi tergantung pada cahaya-6-fosfat dehidrogenase glukosa dan tergantung pada cahaya aktivasi +-malat dehidrogenase NADP telah dipelajari dalam ekstrak dimurnikan sebagian kacang (Pisum sativum) kloroplas. Neither partially purified enzyme could be light modulated by washed thylakoids alone. Baik pemurnian parsial enzim dapat dipengaruhi oleh cahaya thylakoids dicuci sendiri. However, a factor (mol. wt. 50 000) was present in the stroma which could, when added to purified enzyme and thylakoid membranes, reconstitute a light-dependent modulation of either glucose-6-phosphate dehydrogenase or NADP + -malate dehydrogenase. Namun, faktor mol. (wt 000. 50) hadir di stroma yang bisa, bila ditambahkan pada pemurnian enzim dan membran tilakoid, menyusun kembali sebuah-tergantung modulasi cahaya baik-6-fosfat dehidrogenase glukosa atau +-malat dehidrogenase NADP. This factor, which we term protein-modulating factor, is distinct from ferredoxin-thioredoxin reductase and from thioredoxin, the factors involved in another scheme for light modulation. Faktor ini, yang kami faktor protein-modulasi panjang, berbeda dari ferredoxin-thioredoxin reductase dan dari thioredoxin, faktor-faktor yang terlibat dalam skema lain untuk modulasi cahaya. The scheme proposed here for light modulation involves electron transfer from Photosystem I to a membrane-bound light-effect mediator and then to the soluble protein modulating factor which modulates chloroplast enzyme activity, probably by reduction of a regulatory disulfide bond. Skema yang diusulkan di sini untuk modulasi cahaya melibatkan transfer elektron dari Fotosistem saya ke mediator efek cahaya yang terikat membran dan kemudian ke protein larut modulasi kloroplas faktor yang memodulasi aktivitas enzim, mungkin dengan cara pengurangan ikatan disulfida peraturan.

Author Keywords: Light modulation; Protein-modulating factor; NADP + -malate dehydrogenase; Glucose-6-phosphate dehydrogenase; Photosystem I; (Pea chloroplast) Author Keywords: Light modulasi; Protein-faktor modulasi; NADP-malat dehidrogenase +;-6-fosfat dehidrogenase Glukosa; Fotosistem I; (Pea kloroplas)

Abbreviations: DTNB, dithionitrobenzoate; PS I, Photosystem I; Chl, chlorophyll; Tricine, N -tris(hydroxymethyl)-methylglycine; Hepes, N -2-hydroxyethylpiperazine- N ′-2-ethanesulfonic acid Singkatan: DTNB, dithionitrobenzoate; PS Aku, Fotosistem I; Chl, klorofil; Tricine, N-tris (hidroksimetil)-methylglycine; Hepes,-2-hydroxyethylpiperazine-N '-N 2-ethanesulfonic asam
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• References • Referensi

Biochimica et Biophysica Acta (BBA) - Bioenergetics Biochimica et Biophysica Acta (BBA) - Bioenergetika
Volume 638, Issue 2 , 14 December 1981, Pages 242-249 Volume 638, Edisi 2 , 14 Desember 1981, Pages 242-249